Home > Fig1, an interleukin 4-induced mouse B cell gene isolated by cDNA representational difference analysis

Fig1, an interleukin 4-induced mouse B cell gene isolated by cDNA representational difference analysis

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Proc. Natl. Acad. Sci. USA Vol. 94, pp. 2507–2512, March 1997 Immunology
Fig1, an interleukin 4-induced mouse B cell gene isolated by cDNA representational difference analysis
CHARLES C. CHU* AND WILLIAM E. PAUL†
*North Shore University Hospital, Department of Medicine, Manhasset, NY 11030; and †Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892
Contributed by William E. Paul, December 26, 1996
ABSTRACT Interleukin 4 (IL-4) is a cytokine that regu- lates growth and differentiation of lymphoid and nonlym- phoid cells. To study the molecular basis of IL-4 function, we used a cDNA subtraction approach based on the represen- tational difference analysis method. This subtractive am- plification technique allowed us to use small amounts of RNA from lipopolysaccharide IL-4-stimulated normal B cells to obtain IL-4-induced genes from these cells. We report here on one such gene, Fig1 (interleukin-four induced gene 1), the first characterized immediate–early IL-4 in- ducible gene from B cells. Fig1 expression is strikingly limited to the lymphoid compartment. B cells, but not T cells or mast cells, express Fig1 in response to IL-4 within 2 hr in a cycloheximide resistant manner. IL-2, IL-5, and Il-6 do not induce Fig1 in culture. Fig1 maps between Klk1 and Ldh3 on mouse chromosome 7, near two loci involved with murine lupus, Sle3 and Lbw5. The Fig1 cDNA sequence encodes a predicted 70-kDa flavoprotein with best homology to the monoamine oxidases, particularly in domains responsible for FAD binding. Interleukin 4 (IL-4) is a multifunctional cytokine that regulates growth and differentiation among many cell types. It was first recognized because of its actions as a comitogen of B cells and as a determinant of immunoglobulin (Ig) class switching specificity of B lymphocytes stimulated with lipopolysaccha- ride (LPS). It is now known to promote B cell survival in culture and to induce transcription and/or expression of a series of genes including the germ-line and mature forms of the Ig and 1 H chains, major histocompatibility complex class II molecules, Thy-1, and CD23 (1). In general, the induction of these genes by IL-4 is relatively slow (2 days), although germ-line 1 H chain (I1) transcripts have been observed within 4 hr of culture of normal B cells with IL-4 (2). Genes induced earlier in the response to IL-4 have not been charac- terized in this or any other cell type. To understand the molecular basis of IL-4 function, an analysis of such ����early���� genes would be most valuable. To identify IL-4-induced genes in normal B cell cultures, we used a subtractive cDNA hybridization and amplification method based on genomic representational difference analysis (RDA) (3). We report here the isolation and extensive char- acterization of one such gene, Fig1, a putative flavoprotein with homology to monoamine oxidase (MAO). Fig1 is induced in resting B cells within 2 hr in response to IL-4 alone; its induction was not inhibited by cycloheximide. Thus, it qualifies as an immediate–early IL-4-inducible gene.
MATERIALS AND METHODS
Mice. Female 6–16-week-old BALB/c mice, obtained from the Frederick Cancer Research and Development Center, National Cancer Institute (Frederick, MD), were maintained and used in experiments under National Institutes of Health guidelines that meet or exceed standards set forth by the National Research Council (4). Cell Culture. As described (5), resting B cells were prepared from the 60–70% Percoll gradient fraction of dispersed splenocytes treated with antibodies [monoclonal rat anti- mouse anti-Thy1.2 (HO-13-4-9) (6), anti-CD5 (53-7.313) (7), anti-CD8 (3.155) (8), and mouse anti-rat (MAR 18.5) (9)] and complement. B cells were cultured in complete RPMI 1640 medium (5) supplemented, depending on the protocol of the experiment, with 20 g/ml LPS (LPS W Escherichia coli 055:B5, Difco), 20 g/ml cycloheximide (Sigma), 400 units/ml recombinant human IL-2 (gift from Steven Rosenberg, Na- tional Cancer Institute, Bethesda), and the following baculo- virus-derived recombinant mouse cytokines: 100–10,000 units/ml IL-4 (10), 15 ng/ml (150 units/ml) IL-5 (R & D Systems), and 1000 units/ml IL-6 (Genzyme). RNA Preparation. Total RNA was prepared using a guani- dine thiocyanate protocol (11) or a similar RNAzol (Biotecx Laboratories, Houston) protocol. Briefly, 2 107 to 1 108 cultured cells or homogenized tissue from 1–2 mice were lysed in guanidine thiocyanate solution, extracted with a phenol/ chloroform solution, and then precipitated with isopropanol. The resulting RNA was resuspended in diethyl-pyrocarbonate- treated H2O and quantitated by UV absorption measurements at 260 nm. cDNA RDA Subtractive Amplification. Amplicons were prepared from poly(A) mRNA isolated from B cells cultured for 12 and 36 hr with LPS (driver) or LPS and IL-4 (tester) by converting to cDNA, digesting with Sau3AI restriction en- zyme, ligating adaptor sequences to ends of Sau3AI fragments, filling in ends, and amplifying by PCR using a primer with sequence complementary to the adaptor (unpublished work). Driver amplicons were modified by PCR amplification with biotinylated primer. Tester amplicons were modified by re- placing adaptor with a different adaptor sequence. For the first round of subtraction, a 20:1 ratio of driver to tester amplicon (combined 12 and 36 hr) was denatured and hybridized together. After complete hybridization, driver- containing hybrids were removed by streptavidin magnetic beads and tester hybrids were RDA amplified by PCR with primers specific for the tester adaptor, resulting in RDA amplicon 1. RDA amplicon 1 was modified by replacing the adaptors with yet another adaptor sequence. To this, a 100-fold
The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked ����advertisement���� in accordance with 18 U.S.C. ��1734 solely to indicate this fact. Copyright 1997 by THE NATIONAL ACADEMY OF SCIENCES OF THE USA 0027-8424/97/942507-6$2.00/0 PNAS is available online at http://www.pnas.org.
Abbreviations: IL, interleukin; RDA, representational difference analysis; LPS, lipopolysaccharide; EF, elongation factor; MAO, mono- amine oxidase; TMO, tryptophan 2-monooxygenase; PDS, phytoene desaturase; FRD/SDH, fumarate reductase/succinate dehydroge- nase; RACE, rapid amplification of cDNA ends. Data deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. U70429, U70430, and U80211).
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excess of biotinylated driver amplicon was added for the second round of subtraction. Driver-containing hybrids were depleted and tester hybrids were RDA amplified as above, resulting in RDA amplicon 2. After cloning this material, the sequence of 154 individual clones revealed 37 different genes, including Fig1. Two different cDNA RDA subtractive ampli- fication clones, 20-8T#17 and 20-8T#22, cover overlapping parts of Fig1ps and Fig1, respectively. cDNA Library Clones. A gt11 cDNA library made from random-primed cDNA prepared from CBA/J resting splenic B cells stimulated with 300 units/ml of IL-4 for 18 hr (CLON- TECH) was used to isolate nearly full-length cDNA clones of Fig1. PCR product (50 ng), containing the insert of subtraction clone 20-8T#22 (505 bp), was labeled with [-32P]dCTP (3000 Ci/mmol, 1 Ci 37 GBq; Amersham) using an oligolabeling kit (Pharmacia) and used to probe for Fig1 cDNA clones. After three rounds of plaque hybridization according to the manu- facturer��s directions, four clones were isolated: 20-11T1, 20- 11T2, 20-11T3, and 20-11T4. Phage DNA was prepared from each clone according to manufacturer��s instructions and insert cDNA was subcloned into pBluescript SK (Stratagene). A 3.5-kb BsiWI–PvuI fragment from 20-11T1 was ligated to Asp718I-digested pBluescript SK resulting in a linear frag- ment. This fragment was blunt-ended with T4 DNA polymer- ase (Boehringer Mannheim) according to manufacturer��s pro- tocol and then circularized by ligation, resulting in plasmid 20-12R1-9. A 0.6-kb BsiWI fragment from 20-11T2 was ligated into the Asp718I site of pBluescript SK, resulting in plasmid 20-12C2-1. A 2.0-kb BsiWI fragment from 20-11T3 was ligated into the Asp718I site of pBluescript SK, resulting in plasmid 20-12C3-5. A 4.8-kb BsiWI–HindIII fragment from 20-11T4 was ligated into the Asp718I–HindIII sites of pBluescript SK, resulting in plasmid 20-12T4-2. RACE (Rapid Amplification of cDNA Ends). To obtain the 5 end of the Fig1 cDNAs, we performed 5 RACE using the 5-AmpliFINDER RACE kit (CLONTECH). First-strand cDNA was prepared from 1 g poly(A) RNA from LPSIL- 4-stimulated B cells (12 36 hr). The AmpliFINDER anchor oligonucleotide was ligated to the 3 end of the cDNA using RNA ligase in a 10-l reaction. This and other oligonucleotides used in this work are listed in Table 1. An aliquot (1 l of 1:10 dilution) of this material was amplified by PCR in a 50-l reaction containing 50 mM KCl, 22.5 mM Tris (pH 9.0), 0.2 mM dNTP, 2.0 mM MgCl2, 1 M CCC53 or CCC54 primer, 1 M anchor primer, 2.5 units Taq DNA polymerase, and 0.00625 unit Pfu DNA polymerase (Stratagene) in a GeneAmp PCR system 9600 thermal cycler under the following condi- tions: 94C for 30 sec, 35 cycles of [94C for 5 sec, 55C for 15 sec, 72C for 1 min], 72C for 2 min, and a final soak at 4C. This material was diluted 106-fold and 1 l reamplified with a nested primer CCC55, CHU44, or CCC58 and anchor primer or CCC56 as above, except with a higher annealing tempera- ture (63C or 65C). The obtained PCR products were ana- lyzed by 1% agarose gel electrophoresis and products greater than 200 bp were cut out from the gel and purified (12). Purified PCR product was ligated into TA cloning vector pCR3 (Invitrogen); the resulting RACE clones obtained are shown in Table 2. Sequencing from Plasmid or PCR Product. Plasmid DNA was prepared from 4.5-ml cultures of individual clones by a modified mini-alkaline lysis/polyethylene glycol precipitation procedure recommended by Applied Biosystems (Perkin– Elmer). Alternatively, plasmid DNA was prepared by a Qiagen Plasmid Midi kit (Qiagen, Chatsworth, CA) involving a similar alkaline lysis procedure followed by binding to and elution from a plasmid DNA binding column. Plasmid DNA was initially sequenced using United States Biochemical (Amersham) Sequenase Version 2.0 DNA Se- quencing kit with [-33P]dATP (Amersham) and appropriate oligonucleotide primer. Primers CCC32, CCC34, CCC52, NEB#1218, and NEB#1222 were used in the preliminary sequencing of cDNA library subclones. Sequence reactions were electrophoresed through 6% or 8% polyacrylamide se- quencing gels, which were then dried down, autoradiographed, and read. Plasmid DNA of cDNA library subclones, 20-12R#1-9 and 20-12C#3-5 was sequenced initially with CCC34 and CCC35 primers or NEB#1218 and NEB#1222 primers, respectively, by Paragon Biotechnology on a 373 Stretch Sequencer (Ap- plied Biosystems) using an ABI PRISM Dye Terminator Cycle Sequencing Ready Reaction kit with AmpliTaq DNA poly- merase FS (Applied Biosystems Part Number 402122). The sequence of these cDNA library subclones was completed by primer walking (sequences available on request). RACE clones were sequenced from purified PCR product. PCR product was prepared from boiled bacteria (unpublished work) using CCC35 and CCC57 primers and purified through Chromaspin-100 columns (CLONTECH). Purified PCR prod-
Table 1. Oligonucleotides Name* Sequence (5 3 3)† Source‡ AmplifFINDER anchor cacgaattcaCTATCGATTCTGGAACCTTCAGAGG CLONTECH Anchor primer ctggttcggcccaCCTCTGAAGGTTCCAGAATCGATAG CLONTECH CCC32 (SK primer) TCTAGAACTAGTGGATC LI CCC34 (T3 primer) AATAACCCTCACTAAAG LI CCC35 (T7 primer) AATACGACTCACTATAG LI CCC52 (Fig1 524–542) cggaattcggtaccGGAGAAGATGCCAGAAAAG Paragon CCC53 (Fig1ps 1165–1149) cggaattcggtacCACAAGACTCTTGGGGG Paragon CCC54 (Fig1 790–773) cggaattcggtacCGTAAGGCTTCTGCAAAG Paragon CCC55 (Fig1 610–588) cgtgatcaagcTTGAGTGCCATCTGGTAGATGTC Bio-Synthesis CCC56 CCTCTGAAGGTTCCAGAATCGATAG Bio-Synthesis CCC57 (SP6 primer) ATTTAGGTGACACTATAG Bio-Synthesis CCC58 (Fig1 410–393) GTGAGAGCTGGGCATTCG Bio-Synthesis CHU3 (Fig1ps 2213–2194) CCTCGGACATCACATCTCCC Paragon CHU39 (Fig1ps 1711–1730) GGGCCAGTAGAGGTGGGACT Paragon CHU44 (Fig1ps 811–789) AGGAGTCGGGGTGGGGATAGGGG Paragon gt11 forward primer GGTGGCGACGACTCCTGGAGCCCG NEB#1218 gt11 reverse primer TTGACACCAGACCAACTGGTAATG NEB#1222 *Oligonucleotides complementary to Fig1 or partially spliced Fig1 (Fig1ps) have sequence coordinates shown.
†Lowercase sequences indicate added restriction site tails for CCC52, CCC53, CCC54, and CCC55. AmpliFINDER anchor
and anchor primer lowercase letters indicate sequences not complementary to each other.
‡Oligonucleotides were synthesized in the Laboratory of Immunology (LI) as described (5) or purchased from CLONTECH,
Paragon Biotechnology, Bio-Synthesis (Lewisville, TX), or New England Biolabs.
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uct (100 ng/l) was sequenced as before by Paragon Biotech- nology with the same primers used to generate the PCR product. Sequence Analysis. With the aid of the VAXcluster at the Frederick Biomedical Supercomputing Center (National Can- cer Institute, Frederick, MD) and the GCG computer package (Genetics Computer Group, Madison, WI), the full-length Fig1 cDNA sequence was assembled. Coding sequence was predicted by CODONUSE program (Conrad Halling, personal communication). Regions of Fig1 protein structure and ho- mology were determined using the GCG package including
PROFILESCAN (13), supported BLAST (14) service provided by
the National Center for Biotechnology Information (Be- thesda), and FASTA program (15) on the VAXcluster, and
BLOCKS on the internet (http://www.blocks.fhcrc.org) (16).
Comparisons were made to GenBank (release 97.0), EMBL (release 47.0), and Swiss-Prot (release 34.0) databases. Northern Blot Analysis. Denatured RNA samples were electrophoresed in 1% agarose/formaldehyde gels and North- ern blotted to supported nitrocellulose membrane (Optitran, Schleicher & Schuell) by downward capillary method (12). After baking for 1 hr at 80C in a vacuum oven, membranes were prehybridized at 42C in prehyb buffer [40% deionized formamide (Fluka)/4 SSC/10 mM Tris (pH 7.5)/2 Den- hardt��s solution/0.1 mg/ml denatured salmon sperm DNA] for 1 hr and then hybridized overnight at 42C to radioactive- labeled probe in hyb buffer (same as prehyb buffer except it includes 10% dextran sulfate). Probes used were either from cDNA RDA subtraction clone 20-8T#22 (Chromaspin-100- purified PCR product of the Fig1 insert) or clone 20-8T#40 [gel-purified 400-bp Sau3AI-digested PCR product containing the elongation factor-2 (EF-2) insert] labeled with [-32P]dCTP as above (cDNA Library Clones). Membranes were washed two times in 2 SSC/0.1% SDS at 42C for 15 min and two times in 0.1 SSC/0.1% SDS at 65C for 30 min. After air drying, membranes were autoradiographed by expo- sure to x-ray film (Kodak XAR-2) with intensifying screens at 70C from 19 hr to 11 days. Genetic Mapping. To map the genetic location of Fig1, we used the BSS [(C57BL/6JEi Mus spretus SPRET/Ei) SPRET/Ei] Backcross DNA Panel Map Resource (The Jack- son Laboratory) (17). We identified a polymorphism between C57BL/6 and M. spretus by sequencing a 607-bp PCR product that spans an intron–exon border. PCR was performed in two 20-l reactions containing 50 ng M. spretus genomic DNA, 50 mM KCl, 22.5 mM Tris (pH 9.0), 0.2 mM dNTP, 1.5 mM MgCl2, 1 M CHU3 primer, 1 M CHU39 primer, 2.5 units Taq DNA polymerase, and 0.00625 unit Pfu DNA polymerase in a GeneAmp PCR system 9600 thermal cycler under the following conditions: 94C for 30 sec, 35 cycles of [94C for 5 sec, 64C for 15 sec, 72C for 30 sec], 72C for 2 min, and a final soak at 4C. The PCR reactions were combined with 10 l of 10 mM Tris/1 mM EDTA (pH 8.0), purified through Chro- maspin-100 columns, and sequenced by Paragon Biotechnol- ogy using CHU3 and CHU39 primers. Comparison of M. spretus sequence (GenBank accession no. U80211) to Fig1ps sequence revealed HphI and BfuI restriction enzyme site polymorphisms in this fragment. This polymorphism was con- firmed to exist between M. spretus and C57BL/6 by restriction enzyme digestion of PCR product generated from genomic DNA using CHU3 and CHU39 primers. The HphI polymor- phism was used to type PCR product generated from each sample in the BSS Backcross DNA Panel using CHU3 and CHU39 primers. The typing results were then sent to The Jackson Laboratory for determination of mapping position.
RESULTS
Subtraction. To isolate IL-4-induced genes from a subtrac- tion between small populations of normal mouse B lympho- cytes with very slight differences, we developed a PCR method of subtraction based on genomic RDA (3), which we combined with a physical separation method using magnetic beads (unpublished work). This cDNA RDA technique was used to isolate genes expressed in BALB/c splenic B cells cultured in LPS and IL-4 after subtraction from cDNA obtained from B cells cultured with LPS only. Of the 37 genes obtained, three were strongly induced by IL-4: the previously characterized immunoglobulin C1 gene fragment, the mouse homolog of human transcription factor E4BP4, and an unknown gene with no sequence homology to the public databases. Fig1 Gene. We have further characterized this unknown gene originally called clone 20-8T#17/22, which we have renamed Fig1 (interleukin-four induced gene 1). Northern blot analysis of RNA from B cells stimulated with LPS IL-4 confirmed that Fig1 is induced by IL-4 (data not shown). Fig1 probes hybridize to mRNAs of 2.0 and 3.5 kb. The full-length cDNA sequences of both Fig1 mRNAs were obtained and shown schematically in Fig. 1. The sequence of the larger mRNA reveals that it is a partially spliced pre- mRNA (Fig1ps) that still contains two introns. The first exon
Table 2. RACE clones First round primers Second round primers RACE clones Insert, bp CCC53/anchor CHU44/anchor 20-13#T20 722 20-13#T49 588 CCC54/anchor CCC55/anchor 20-13#M38 313 20-13#M45 294 CCC53/anchor CCC58/CCC56 20-13#V5 443 20-13#V8 435 CCC54/anchor CCC58/CCC56 20-13#U20 234 20-13#U28 269 20-13#U29 445 FIG. 1. Schematic of both 2.0- and 3.5-kb Fig1 mRNA sequences. The sequences of two distinct Fig1 mRNAs were largely obtained from cDNA library clones and the remaining 5 end sequences were obtained by RACE. Both mRNA forms are shown schematically with coding regions shown in large solid rectangles and 5 and 3 untrans- lated regions in large shaded rectangles; areas corresponding to introns and to the poly(A) tail are shown in thin rectangles. Exons and introns of Fig1ps are numbered. Locations of original subtraction library clones 20-8T#17 and 20-8T#22 are indicated. Clone 20-8T#17 contains exon 2 and part of intron 2 from Fig1ps. Clone 20-8T#22 contains exon 2 and part of exon 3 from Fig1. , splice sites, with one indicating a splice site found in the 607-bp genomic sequence obtained for genetic mapping. , Sau3AI restriction enzyme sites. Lengths in bp are shown in parentheses. Fig1 (GenBank accession no. U70429) and Fig1ps (GenBank accession no. U70430) had slight sequence differences (7 bp) in the untranslated area close to the poly(A) tail.
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could possibly encode a short protein of 13 kDa, which would have no additional coding sequence contributed by the first intron due to an immediate translational stop codon found in the intron. Fig1ps does not represent genomic DNA since some introns have been spliced out. A 607-bp genomic sequence revealed that a short intron in exon 3 has been spliced out (Fig. 1 and genetic mapping). Homology to MAO (see below) suggests many more introns have been removed. Thus, Fig1ps is unlikely to produce a functional protein and most likely represents a partially spliced pre-mRNA. The sequence of the shorter mRNA (Fig1) reveals a single large open reading frame with good codon usage that encodes a 630 amino acid protein with a predicted mass of 70 kDa. The full-length sequence contains some amino acid homology to many proteins. These homologies were not found in the sequence of the two original cDNA RDA subtraction library clones 20-8T#17 and 20-8T#22. The predicted Fig1 protein contains a putative signal peptide sequence for translation into the endoplasmic reticulum and hence may possibly be secreted (Fig. 2). It contains three possible N-glycosylation sites and two possible tyrosine phos- phorylation sites. The protein is mostly hydrophilic with five pockets of hydrophobic nature (including the signal peptide sequence). The strongest homology is found with proteins that bind FAD or NAD as a cofactor. Five areas (1–5), which may be involved in FAD binding are illustrated in Fig. 2: (i) binds the ADP moiety of FAD based on homology to a host of FAD and NAD binding proteins that contain the consensus se- quence for the dinucleotide binding fold (18); (ii) binds the ribityl moiety of FAD based on homology to MAO (19); (iii) may bind to an unknown portion of FAD based on its identification by the PROFILESCAN program and its homology to MAO; (iv) may bind to another unknown region of FAD based on its identification by PROFILESCAN and its homology to phytoene desaturase (PDS); and (v) probably binds the flavin component of FAD based on homology to MAO (20). Of these five areas, the homologies in iii and iv have not been clearly defined as FAD binding regions. The best homologies were found with MAO (23% identical over 460 amino acids), tryptophan 2-monooxygenase (42% identical over 92 amino acids), and PDS (38% identical over 80 amino acids). Another weak area of homology to the active site of the fumarate reductase/succinate dehydrogenase (FRD/SDH) family of proteins (26% over 47 amino acids) (21) was uncovered by the
BLOCKS program. Although most similar to MAO, Fig1 is not
MAO, because it does not contain the MAO conserved cysteine residue used to covalently bind FAD, its splice sites are in different locations, and despite five large areas of homology (Fig. 2), there are large stretches of nonhomologous amino acids in contrast to rat and human MAO, which are 88% identical over 519 amino acids. Based on these homologies, Fig1 appears to be an enzymatic flavoprotein with a possible active site similar to FRD/SDH. Fig1 Genetic Mapping. Fig1 was genetically mapped using The Jackson Laboratory BSS Backcross DNA Panel Mapping Service to the proximal portion of mouse chromosome 7 between the Klk1 and Ldh3 markers (Fig. 3). Human Fig1 is deduced to reside on chromosome 19q13.3-q13.4 because mouse Fig1 is located between genetic markers that are syntenic with this portion of human chromosome 19 (see The Jackson Laboratory BSS backcross mapping data at the World Wide Web address http://www/jax/org/resources/documents/ cmdata). Interestingly, several genetic traits that affect anti- body responses map to this region. In the SAM-P/1 mouse, one of two genes responsible for the Lar (low antibody response) trait maps to the proximal portion of chromosome 7 (22). In the mouse (NZW NZB)F1 hybrid lupus model, two susceptibility genes, Lbw5 and Sle3, map to this region (23, 24).
FIG. 2. Schematic of predicted Fig1 protein. The results of com- puter program-aided sequence analysis of Fig1 is summarized in schematic form. Possible N-linked glycosylation sites (N-Gly) and tyrosine phosphorylation sites (Y) are indicated. Locations of se- quence inferred from original subtraction library clones 20-8T#17 and 20-8T#22 are indicated. Predicted hydrophobic regions and FAD cofactor-binding regions (numbered 1–5) are shown. In addition, regions homologous to many known FAD binding and NAD binding proteins (MANY) and to specific FAD binding proteins (MAO, PDS, TMO, and FRD/SDH) are shown. The predicted signal peptide domain is also indicated (SIGNAL). , Location of the homologous FRD/SDH active site. The consensus sequence for the dinucleotide binding fold (FAD-binding region 1) is -#-X-#-G-X-G-X-X-G-X- X-X-#-X-X-#-X-X-X-X-X-X-#-X-#-X-. , a hydrophilic, posi- tively charged residue; #, a hydrophobic residue; X, any amino acid; G, glycine; , an acidic residue. FIG. 3. Genetic mapping. Haplotype figure from The Jackson Laboratory BSS backcross showing part of chromosome 7 with loci linked to Fig1. Loci are listed in order, with the most proximal at the top. Solid boxes represent the C57BL6/JEi allele and open boxes the SPRET/Ei allele. The number of animals with each haplotype is given at the bottom of each column of boxes. The percent recombination (R) between adjacent loci, equivalent to centimorgans, is given to the right of the figure, with the standard error (SE) for each R. FIG. 4. Tissue expression of Fig1. Total RNA (10 g) prepared from the indicated mouse tissues was electrophoresed, Northern blotted, and probed with Fig1 or EF-2 (internal mRNA ����housekeep- ing���� standard). Ethidium bromide stained 28S and 18S rRNA bands are shown for comparison.
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Fig1 Expression. Expression of Fig1 is strikingly limited to lymphoid tissues (Fig. 4), with highest expression in lymph node and spleen. A trace amount of Fig1 is detectable in thymus upon longer exposure. In B cells, Fig1 is induced specifically by IL-4 and not by several other type I cytokines (IL-2, IL-4, IL-5, and IL-6). This induction does not require LPS (Fig. 5). Fig1 is induced within the first 2 hr and remains elevated for at least 36 hr (Fig. 5). Fig1 induction by IL-4 is cycloheximide resistant implying that new protein synthesis is not required for its induction (Fig. 6). Thus, Fig1 is the first immediate–early IL-4-inducible gene characterized in B cells. Fig1 is not expressed in naive T cells; there may be some very weak Fig1 expression in response to IL-4 in primed TH1 and TH2 cells (data not shown). Mast cells treated with or without IL-4 also do not express Fig1 (data not shown). Thus, Fig1 appears to be a largely B cell-specific IL-4-inducible gene.
DISCUSSION
We have isolated Fig1 from mouse B cells by the cDNA RDA method, a PCR-based subtraction technique. Fig1 expression has thus far been observed only in lymphoid tissues and appears to be largely limited to expression to B cells. It is induced within 2 hr in normal mouse B cells and its induction is cycloheximide resistant. Thus, it is the first characterized IL-4-dependent immediate–early gene. Initial studies using a mouse B cell line transfected with various mutants of the human IL-4 receptor indicate that the region of the receptor required for STAT6 activation is required for upregulating Fig1 expression (C.C.C., J. J. Ryan, and W.E.P., unpublished results). This is consistent with requirements for induction of other IL-4-dependent genes but differs from requirements for IL-4-mediated cell growth (25). Is Fig1 only inducible by IL-4? IL-2, IL-5, and IL-6 did not induce Fig1 in culture. However, we have not tested a variety of other factor combinations in vitro. Indeed, Fig1 mRNA expression in lymph nodes of IL-4 knockout mice was similar to that of the wild type (unpublished results), indicating the existence of an IL-4-independent mechanism for induction of Fig1. This is probably not via cytokines that signal through receptors that contain the c chain (IL-2, IL-7, IL-9, IL-15), because the IL-2 receptor includes the c chain in common with the IL-4 receptor. Whether such Fig1 expression requires the IL-4 receptor chain, possibly activated by IL-13, remains to be established. Other receptor systems that signal via STAT6 (platelet-derived growth factor, leptin, B cell antigen receptor) may be involved (25). However, the possibility of a totally different signaling system for Fig1 induction must be considered. Genetic mapping located Fig1 to a region of mouse chro- mosome 7 that contains Lbw5 and Sle3, which are involved in the lupus-like disease found in (NZB NZW)F1 hybrid mice. Because two different groups (23, 24) identified this mutation in the same region, Lbw5 and Sle3 are likely to be the same gene. The idea that this lupus-associated locus is the same as Fig1 is attractive, because Fig1 is expressed in antibody- producing B cells induced with IL-4 (a regulator of the humoral immune response). Preliminary analysis of the induc- tion by IL-4 and size of Fig1 transcripts in B cells of (NZB NZW)F1 hybrid and Sle3 affected mice (24) show no notice- able alterations (L. M. Morel, C.C.C., W.E.P., and E. K. Wakeland, unpublished results). However, the possibility still exists that Fig1 may be mutated at the protein level in these mice. The function of Fig1 remains to be solved. Its homologies suggest that it is a secreted FAD binding protein. From its similarity to MAO, it is tempting to speculate that Fig1 may also inactivate monoamine transmitters in a similar manner to the MAO inactivation of serotonin. This is particularly pro- vocative in view of the role IL-4 plays in allergic inflammatory responses.
We thank Hangjiong Chen, Cyndy Watson, Carol Kinzer, S. Z. Ben-Sasson, Jane Hu-Li, and Hua Huang for help in preparing cell cultures and RNA, Lucy Rowe for the mapping figure, Michail Sitkovsky for cycloheximide, Shirley Starnes for preparation of this manuscript, and Keats Nelms and Ronald Germain for helpful dis- cussion. 1. Paul, W. E. (1991) Blood 77, 1859–1870. 2. Berton, M. T., Uhr, J. W. & Vitetta, E. S. (1989) Proc. Natl. Acad. Sci. USA 86, 2829–2833. 3. Lisitsyn, N., Lisitsyn, N. & Wigler, M. (1993) Science 259, 946–951. 4. National Research Council (1985) Guide for the Care and Use of Laboratory Animals, NIH Publication 85-23 (U.S. Public Health Service, Bethesda). FIG. 5. Fig1 induced by IL-4 specifically. Total RNA (10 g) prepared from resting BALB/c splenic B lymphocytes (0 hr) or from B lymphocytes stimulated with IL-4 alone (1000 units/ml) for 1, 2, 6, and 12 hr (A), or with IL-4 alone (1000 units/ml), IL-2 alone, LPS alone, or LPS plus IL-4, IL-5, or IL-6 for 36 hr (B) was electropho- resed, Northern blotted, and probed with Fig1 or EF-2. Ethidium bromide stained 28S and 18S rRNA bands are shown for comparison. Concentrations of other reactants have been described. FIG. 6. Fig1 induction by IL-4 in B cells is not inhibited by cycloheximide. Total RNA (8.2 g) prepared from BALB/c splenic B lymphocytes after culture for 2 or 3 hr under the conditions indicated [IL-4 (1000 units/ml), cycloheximide (CHX)] was electrophoresed, Northern blotted, and probed with Fig1 or EF-2. Ethidium bromide stained 28S and 18S rRNA bands are shown for comparison.
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2512 Immunology: Chu and Paul Proc. Natl. Acad. Sci. USA 94 (1997)

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